KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
pyridoxamine 5'-phosphate | pH 8.0 | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
native enzyme 556fold from liver by acid and ethanol precipitations, anion exchange chromatography, gel filtration, and calcium phosphate gel chromatography | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 | reaction mechanism | Oryctolagus cuniculus | |
pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2 | reaction mechanism | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.156 | - |
- |
Oryctolagus cuniculus |
0.157 | - |
purified enzyme | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-phospho-pyridoxyl-2-aminobutyrate + H2O + O2 | 110% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + 2-aminobutyrate + H2O2 | - |
? | |
5'-phospho-pyridoxyl-3-alanine + H2O + O2 | 85% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + alanine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-benzylamine + H2O + O2 | 120% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + benzylamine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-D-2-aminobutyrate + H2O + O2 | 120% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + D-2-aminobutyrate + H2O2 | - |
? | |
5'-phospho-pyridoxyl-D-alanine + H2O + O2 | 130% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + D-alanine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-D-leucine + H2O + O2 | 120% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + D-leucine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-D-tyrosine + H2O + O2 | 55% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + D-tyrosine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-glycine + H2O + O2 | 110% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + glycine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-2-aminobutyrate + H2O + O2 | - |
Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-2-aminobutyrate + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-alanine + H2O + O2 | 140% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-alanine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-leucine + H2O + O2 | 86% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-leucine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-phenylalanine + H2O + O2 | 54% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-phenylalanine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-serine + H2O + O2 | 39% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-serine + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-tryptophan + H2O + O2 | 18% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-tryptophan + H2O2 | - |
? | |
5'-phospho-pyridoxyl-L-tyrosine + H2O + O2 | 57% of activity with pyridoxamine 5'-phosphate | Oryctolagus cuniculus | pyridoxal 5'-phosphate + L-tyrosine + H2O2 | - |
? | |
additional information | a 5'-phosphate moiety is absolutely required for activity, substrate specificities with various N-(phosphopyridoxyl)amines, overview, no activity with pyridoxamine, N-(pyridoxyl)-glycine, N-(pyridoxyl)-S-alanine, or N-(pyridoxyl)-L-tyrosine | Oryctolagus cuniculus | ? | - |
? | |
N10-(5'-phosphopyridoxyl)-1,10-diaminodecane + H2O + O2 | - |
Oryctolagus cuniculus | pyridoxal 5'-phosphate + 1,10-diaminodecane + H2O2 | - |
? | |
pyridoxamine 5'-phosphate + H2O + O2 | - |
Oryctolagus cuniculus | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
pyridoxine 5'-phosphate + O2 | - |
Oryctolagus cuniculus | pyridoxal 5'-phosphate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyridoxamine (pyridoxine) 5'-phosphate oxidase | - |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Oryctolagus cuniculus |